/lab/aaron-whiteley/ en /lab/aaron-whiteley/2019/05/02/analysis-human-cgas-activity-and-structure <span>Analysis of human cGAS activity and structure</span> <span><span>Anonymous (not verified)</span></span> <span><time datetime="2019-05-02T00:00:00-06:00" title="Thursday, May 2, 2019 - 00:00">Thu, 05/02/2019 - 00:00</time> </span> <div> <div class="imageMediaStyle focal_image_wide"> <img loading="lazy" src="/lab/aaron-whiteley/sites/default/files/styles/focal_image_wide/public/article-thumbnail/screen_shot_2020-08-01_at_3.56.52_pm_0.png?h=bae1b1a2&amp;itok=3p2_Xyyt" width="1200" height="800" alt="Fig 1 of Methods Paper"> </div> </div> <div role="contentinfo" class="container ucb-article-tags" itemprop="keywords"> <span class="visually-hidden">Tags:</span> <div class="ucb-article-tag-icon" aria-hidden="true"> <i class="fa-solid fa-tags"></i> </div> <a href="/lab/aaron-whiteley/taxonomy/term/169" hreflang="en">DNA sensing</a> <a href="/lab/aaron-whiteley/taxonomy/term/159" hreflang="en">STING</a> <a href="/lab/aaron-whiteley/taxonomy/term/175" hreflang="en">X-ray crystallography</a> <a href="/lab/aaron-whiteley/taxonomy/term/167" hreflang="en">biochemistry</a> <a href="/lab/aaron-whiteley/taxonomy/term/163" hreflang="en">cGAS</a> <a href="/lab/aaron-whiteley/taxonomy/term/171" hreflang="en">genetic screen</a> <a href="/lab/aaron-whiteley/taxonomy/term/157" hreflang="en">innate immunity</a> <a href="/lab/aaron-whiteley/taxonomy/term/173" hreflang="en">nucleotidyltransferase</a> </div> <span>Zhou W</span> <span>,&nbsp;</span> <span>鉃hiteley AT</span> <span>,&nbsp;</span> <span>Kranzusch PJ</span> <div class="ucb-article-content ucb-striped-content"> <div class="container"> <div class="paragraph paragraph--type--article-content paragraph--view-mode--default"> <div class="ucb-article-content-media ucb-article-content-media-above"> <div> <div class="paragraph paragraph--type--media paragraph--view-mode--default"> <div> <div class="imageMediaStyle large_image_style"> <img loading="lazy" src="/lab/aaron-whiteley/sites/default/files/styles/large_image_style/public/article-image/screen_shot_2020-08-01_at_3.56.52_pm.png?itok=LkLikqTo" width="1500" height="1500" alt="Fig 1 of Methods Paper"> </div> </div> </div> </div> </div> <div class="ucb-article-text d-flex align-items-center" itemprop="articleBody"> <div><p><em>Methods Enzymol.</em>&nbsp;2019;625:13-40. doi: 10.1016/bs.mie.2019.04.012. Epub 2019 May 2.</p> <h3>Abstract</h3> <p>Cyclic GMP-AMP synthase (cGAS) is an innate immune system enzyme responsible for recognition of double-stranded DNA aberrantly localized in the cell cytosol. cGAS binds DNA and is activated to catalyze production of the nucleotide second messenger 2'-5'/3'-5' cyclic GMP-AMP (2'3' cGAMP). In spite of a major role for cGAS in the cellular immune response, a complete understanding of cGAS biology has been limited by a lack of genetic tools to rapidly screen cGAS activity, instability of human cGAS-DNA interactions in vitro, and a previous absence of structural information for the human cGAS-DNA complex. Here we detail procedures to map the molecular determinants of cGAS activation and describe methods developed to prepare human cGAS-DNA crystals for structural analysis. Together with earlier systems established to study mammalian homologs of cGAS, these innovations provide a foundation to understand and therapeutically target human cGAS biology.</p> <h3>Links</h3> <ul> <li> <p>PMID:<a href="https://www.ncbi.nlm.nih.gov/pubmed/31455523/" target="_blank" rel="nofollow">31455523</a></p> </li> <li> <p>DOI:<a href="https://doi.org/10.1016/bs.mie.2019.04.012" target="_blank" rel="nofollow">10.1016/bs.mie.2019.04.012</a></p> </li> </ul></div> </div> </div> </div> </div> <div>Zhou W, 鉃hiteley AT, Kranzusch PJ. | Methods Enzymol. 2019</div> <h2> <div class="paragraph paragraph--type--ucb-related-articles-block paragraph--view-mode--default"> <div>Off</div> </div> </h2> <div>Traditional</div> <div>0</div> <div>On</div> <div>White</div> Thu, 02 May 2019 06:00:00 +0000 Anonymous 29 at /lab/aaron-whiteley